Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.

Osorio, José Henry

Titulo:

Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
.

Sumario:

Antecedentes: la toma de muestras de sangre es un factor importante que puede influir los resultados finales de algunas pruebas de laboratorio. Las determinaciones de acilcarnitinas en sangre son utilizadas corrientemente para el diagnóstico y seguimiento de pacientes que sufren enfermedades metabólicas hereditarias. Objetivo: el presente estudio analizó la posible influencia del tipo de anticoagulante utilizado en la toma de la muestra de sangre, sobre el perfil de acilcarnitinas obtenido. Metodología: muestras de sangre y plasma fueron obtenidas utilizando heparina, potasio-EDTA, fluoruro de sodio-oxalato de potasio, y citrato de sodio y sin anticoagulante. Resultados: no fue encontrada diferencia estadísticamente significativa entre los... Ver más

Guardado en:

Revista:

Biosalud

ISSN:

1657-9550

EISSN:

2462-960X

Autores:

Osorio, José Henry

Editor:

UNIVERSIDAD DE CALDAS

Volumen:

Año de publicación:

2022-03-17

Pagina inicial:

Pagina final:

Pais:

Colombia

Palabras claves:

carnitina

acilcarnitinas

espectrometría de masas en tándem

Licencia:

Revista Biosalud - 2010

info:eu-repo/semantics/openAccess

http://purl.org/coar/access_right/c_abf2

id	oai:revistasojs.ucaldas.edu.co:article_5498
record_format	ojs
spelling	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. Efecto del tipo de anticoagulante utilizado en la toma de muestras, sobre la determinación del perfil de acilcarnitinas en sangre y plasma. Antecedentes: la toma de muestras de sangre es un factor importante que puede influir los resultados finales de algunas pruebas de laboratorio. Las determinaciones de acilcarnitinas en sangre son utilizadas corrientemente para el diagnóstico y seguimiento de pacientes que sufren enfermedades metabólicas hereditarias. Objetivo: el presente estudio analizó la posible influencia del tipo de anticoagulante utilizado en la toma de la muestra de sangre, sobre el perfil de acilcarnitinas obtenido. Metodología: muestras de sangre y plasma fueron obtenidas utilizando heparina, potasio-EDTA, fluoruro de sodio-oxalato de potasio, y citrato de sodio y sin anticoagulante. Resultados: no fue encontrada diferencia estadísticamente significativa entre los anticoagulantes utilizados. Conclusión: el tipo de anticoagulante no tiene efecto sobre la determinación de acilcarnitinas en sangre o plasma. Para este tipo de análisis, puede ser utilizado cualquier anticoagulante de los antes mencionados. Abreviaturas: IEE-MS/MS, inyección de electrospray espectrometría de masas en tándem; EIM, errores innatos del metabolismo. Background: the collection process for blood sampling is a very important factor that can influence the final results of some laboratory tests. The acylcarnitine determinations in blood are usually used for diagnosis and follow up of patients suffering certain inherited metabolic illnesses. Objective: this study analyzed the possible influence of the type of anticoagulant used in blood sampling on the acylcarnitine profile. Methodology: blood and plasma samples were collected using anticoagulants such as: lithium heparin, potassium EDTA, sodium fluoride-potassium oxalate, and sodium citrate. Samples were also collected without the use of anticoagulants. Acylcarnitine levels were assessed for each sample via tandem mass spectrometry. Results: no significant differences were found among the anticoagulant used. Conclusion: the type of anticoagulant has no effect on the acylcarnitine determination on blood or plasma. For this type of analysis any of the above mentioned anticoagulants can be used. Abbreviations: ESI-MS/MS, electrospray injection tandem mass spectrometry; IEM, innate errors of metabolism. Osorio, José Henry carnitina acilcarnitinas espectrometría de masas en tándem carnitine acylcarnitines tandem mass spectrometry 9 2 Núm. 2 , Año 2010 : Julio - Diciembre Artículo de revista Journal article 2022-03-17T00:37:02Z 2022-03-17T00:37:02Z 2022-03-17 application/pdf Universidad de Caldas Biosalud 1657-9550 2462-960X https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498 https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498 eng https://creativecommons.org/licenses/by-nc-sa/4.0/ Revista Biosalud - 2010 9 13 Nagaraja D, Mamatha SN, De T, Christopher R. Screening for inborn errors of metabolism using automated electrospray tandem mass spectrometry: study in high-risk Indian population. Clin Biochem 2010;43(6):581-8. Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000;46(5):727-9. Kodo N, Millington DS, Norwood DL, Roe CR. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1990 Jan 31;186(3):383-90. Osorio JH, Pourfarzam M. Plasma free and total carnitine measured in children by tandem mass spectrometry. Braz J Med Biol Res 2002;35(11):1265-71. Osorio JH, Pourfarzam M. Determination of normal acylcarnitine levels in a healthy pediatric population as a diagnostic tool in inherited errors of mitochondrial fatty acid beta-oxidation. An Pediatr (Barc) 2007;67(6):548-52. Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010;603:9-25. Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010;44(2):15-20. Holub M, Tuschl K, Ratschmann R, Strnadová KA , Mühl A , Heinze G, et al. Influence of hematocrit and localisation of punch in dried blood spots on levels of amino acids and acylcarnitines measured by tandem mass spectrometry. Clin Chim Acta 2006; 373:27–31. Sewell AC. Methods of carnitine analysis. In: Seim H, Löster H, editors. Carnitine-Pathobiochemical Basics and Clinical Applications. Bochum: Ponte Press 1996: 73-84. Schoderbeck M, Auer B, Legenstein E, Genger H, et al. Pregnancy-related changes of carnitine and acylcarnitine concentrations of plasma and erythrocytes. J Perinat Med 1995;23:477-85. Mancinelli A, Iannoni E, Calvani M. Effect of temperature on the stability of long-chain acylcarnitines in human blood prior to plasma separation. Clin Chim Acta 2007; 375:169-170. https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/5498/4965 info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_2df8fbb1 http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Text Publication
institution	UNIVERSIDAD DE CALDAS
thumbnail	https://nuevo.metarevistas.org/UNIVERSIDADDECALDAS/logo.png
country_str	Colombia
collection	Biosalud
title	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
spellingShingle	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. Osorio, José Henry carnitina acilcarnitinas espectrometría de masas en tándem carnitine acylcarnitines tandem mass spectrometry
title_short	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
title_full	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
title_fullStr	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
title_full_unstemmed	Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
title_sort	effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
title_eng	Efecto del tipo de anticoagulante utilizado en la toma de muestras, sobre la determinación del perfil de acilcarnitinas en sangre y plasma.
description	Antecedentes: la toma de muestras de sangre es un factor importante que puede influir los resultados finales de algunas pruebas de laboratorio. Las determinaciones de acilcarnitinas en sangre son utilizadas corrientemente para el diagnóstico y seguimiento de pacientes que sufren enfermedades metabólicas hereditarias. Objetivo: el presente estudio analizó la posible influencia del tipo de anticoagulante utilizado en la toma de la muestra de sangre, sobre el perfil de acilcarnitinas obtenido. Metodología: muestras de sangre y plasma fueron obtenidas utilizando heparina, potasio-EDTA, fluoruro de sodio-oxalato de potasio, y citrato de sodio y sin anticoagulante. Resultados: no fue encontrada diferencia estadísticamente significativa entre los anticoagulantes utilizados. Conclusión: el tipo de anticoagulante no tiene efecto sobre la determinación de acilcarnitinas en sangre o plasma. Para este tipo de análisis, puede ser utilizado cualquier anticoagulante de los antes mencionados. Abreviaturas: IEE-MS/MS, inyección de electrospray espectrometría de masas en tándem; EIM, errores innatos del metabolismo.
description_eng	Background: the collection process for blood sampling is a very important factor that can influence the final results of some laboratory tests. The acylcarnitine determinations in blood are usually used for diagnosis and follow up of patients suffering certain inherited metabolic illnesses. Objective: this study analyzed the possible influence of the type of anticoagulant used in blood sampling on the acylcarnitine profile. Methodology: blood and plasma samples were collected using anticoagulants such as: lithium heparin, potassium EDTA, sodium fluoride-potassium oxalate, and sodium citrate. Samples were also collected without the use of anticoagulants. Acylcarnitine levels were assessed for each sample via tandem mass spectrometry. Results: no significant differences were found among the anticoagulant used. Conclusion: the type of anticoagulant has no effect on the acylcarnitine determination on blood or plasma. For this type of analysis any of the above mentioned anticoagulants can be used. Abbreviations: ESI-MS/MS, electrospray injection tandem mass spectrometry; IEM, innate errors of metabolism.
author	Osorio, José Henry
author_facet	Osorio, José Henry
topicspa_str_mv	carnitina acilcarnitinas espectrometría de masas en tándem
topic	carnitina acilcarnitinas espectrometría de masas en tándem carnitine acylcarnitines tandem mass spectrometry
topic_facet	carnitina acilcarnitinas espectrometría de masas en tándem carnitine acylcarnitines tandem mass spectrometry
citationvolume	9
citationissue	2
citationedition	Núm. 2 , Año 2010 : Julio - Diciembre
publisher	Universidad de Caldas
ispartofjournal	Biosalud
source	https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498
language	eng
format	Article
rights	https://creativecommons.org/licenses/by-nc-sa/4.0/ Revista Biosalud - 2010 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2
references_eng	Nagaraja D, Mamatha SN, De T, Christopher R. Screening for inborn errors of metabolism using automated electrospray tandem mass spectrometry: study in high-risk Indian population. Clin Biochem 2010;43(6):581-8. Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000;46(5):727-9. Kodo N, Millington DS, Norwood DL, Roe CR. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1990 Jan 31;186(3):383-90. Osorio JH, Pourfarzam M. Plasma free and total carnitine measured in children by tandem mass spectrometry. Braz J Med Biol Res 2002;35(11):1265-71. Osorio JH, Pourfarzam M. Determination of normal acylcarnitine levels in a healthy pediatric population as a diagnostic tool in inherited errors of mitochondrial fatty acid beta-oxidation. An Pediatr (Barc) 2007;67(6):548-52. Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010;603:9-25. Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010;44(2):15-20. Holub M, Tuschl K, Ratschmann R, Strnadová KA , Mühl A , Heinze G, et al. Influence of hematocrit and localisation of punch in dried blood spots on levels of amino acids and acylcarnitines measured by tandem mass spectrometry. Clin Chim Acta 2006; 373:27–31. Sewell AC. Methods of carnitine analysis. In: Seim H, Löster H, editors. Carnitine-Pathobiochemical Basics and Clinical Applications. Bochum: Ponte Press 1996: 73-84. Schoderbeck M, Auer B, Legenstein E, Genger H, et al. Pregnancy-related changes of carnitine and acylcarnitine concentrations of plasma and erythrocytes. J Perinat Med 1995;23:477-85. Mancinelli A, Iannoni E, Calvani M. Effect of temperature on the stability of long-chain acylcarnitines in human blood prior to plasma separation. Clin Chim Acta 2007; 375:169-170.
type_driver	info:eu-repo/semantics/article
type_coar	http://purl.org/coar/resource_type/c_6501
type_version	info:eu-repo/semantics/publishedVersion
type_coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85
type_content	Text
publishDate	2022-03-17
date_accessioned	2022-03-17T00:37:02Z
date_available	2022-03-17T00:37:02Z
url	https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498
url_doi	https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498
issn	1657-9550
eissn	2462-960X
citationstartpage	9
citationendpage	13
url2_str_mv	https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/5498/4965
_version_	1811199447451828224

Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. .

Código QR

Estadísticas y Métricas Alternativas

Títulos similares

Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
.