Titulo:
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
.
Sumario:
El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ova... Ver más
Guardado en:
0121-3709
2011-2629
24
2020-05-11
42
51
Orinoquia - 2020
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metarevistapublica_unillanos_orinoquia_57_article_590 |
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Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando Luteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cows El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo. The luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance. Vejarano-Orjuela, Álvaro Hernández-Vásquez, Aureliano Rondón-Barragán, Iang S. Suárez-Mesa, Rafael A. bovine creole reproduction LHR–LHCGR fertility indicator bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR 24 1 Artículo de revista Journal article 2020-05-11T00:00:00Z 2020-05-11T00:00:00Z 2020-05-11 application/pdf Universidad de los Llanos Orinoquia 0121-3709 2011-2629 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/590 10.22579/20112629.590 https://doi.org/10.22579/20112629.590 spa https://creativecommons.org/licenses/by-nc-sa/4.0/ Orinoquia - 2020 42 51 Cadagan D, Towlson C. Mechanisms of Luteinising Hormone Regulation in Female Steroidogenesis. Am J Med, 2017;5:65-68. Casarini L, Pignatti E, Simoni M. Effects of polymorphisms in gonadotropin and gonadotropin receptor genes on reproductive function. Rev Endocr Metab Dis, 2011;12:303. Clelland E, Peng C. Endocrine/paracrine control of zebrafish ovarian development. Mol Cell Endocrinol, 2009;312:42-52. Delemi A. Identificaion of the expression level to LH-r Gene in dominant and cystic ovarian follicles cells of the cow. Al-Delemi DHJ, Al-Gewary AKA, Ali Jeddoa Z. Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows. J Dairy Vet Anim Res, 2014;1(3):80-86. DOI: 10.15406/jdvar.2014.01.00017 Dickinson RE, Stewart AJ, Myers M, Millar RP Duncan WC. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology, 2009;150:2873-2881. Gay V, Midgley Jr A, Niswender G. (1970). Patterns of gonadotrophin secretion associated with ovulation. Federation proceedings, p. 1880. Ginther O. Selection of the dominant follicle in cattle and horses. Anim Reprod Sci, 2000;60:61-79. Hastings N, Donn S, Derecka K, Flint A, Woolliams J. Polymorphisms within the coding region of the bovine luteinizing hormone receptor gene and their association with fertility traits. Anim Genet, 2006;37:583-585. Holdridge LR. 1967. Life zone ecology. Life zone ecology. Johnson A, Bridgham J, Wagner B. Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod, 1996;55: 304-309. Kawate N. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. J Reprod Develop, 2004;50:1-8. Larsson B. Determination of Ovulation by Ultrasound Examination and its Relation to the LH‐Peak in Heifers. J Vet Med A, 1987;34:749-754. Liu KC, Lin SW, Ge W. Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane. Endocrinology, 2011;152:4418-4430. Ma T, Xiong Q, Yuan B, Jiang H, Gao Y, Xu J, Liu S, et al. Luteinizing hormone receptor splicing variants in bovine Leydig cells. Genet Mol Res, 2012;11:1721-1730. Mamluk R, Wolfenson D, Meidan R. LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin. Domest Anim Endocrinol, 1998;15:103-114. McArdle C, Roberson M. Gonadotropes and gonadotropin-releasing hormone signaling. Knobil and Neill’s Physiology of Reproduction, 2015;4:335-397. Nejad SZ, Tehrani FR, Zadeh-Vakili A. The Role of Kisspeptin in Female Reproduction. Int J Endocrinol Metab, 2017;15(3):e44337. doi: 10.5812/ijem.44337. Nogueira M, Buratini J, Price C, Castilho A, Pinto M, Barros C. Expression of LH receptor mRNA splice variants in bovine granulosa cells: changes with follicle size and regulation by FSH in vitro. Mol Reprod Dev, 2007;74: 680-686. Peiró JR, Nogueira GM, Nogueira GP, Perri SH, Cardoso D. Ovariectomy by left flank approach in prepubertal Nelore (Bos indicus) heifers. Can J Vet Res, 2009;73:237-240. Pierson R, Ginther O. Ultrasonic imaging of the ovaries and uterus in cattle. Theriogenology, 1988;29:21-37. Rawan A, Yoshioka S, Abe H, Acosta T. Insulin‐Like Growth Factor‐1 Regulates the Expression of Luteinizing Hormone Receptor and Steroid Production in Bovine Granulosa Cells. Reprod Domest Anim, 2015;50:283-291. Richards J, Hedin L. Molecular aspects of hormone action in ovarian follicular development, ovulation, and luteinization. Annu Rev Physiol, 1988;50: 441-463. Robert C, Gagne D, Lussier J, Bousquet D, Barnes F, Sirard M. Presence of LH receptor mRNA in granulosa cells as a potential marker of oocyte developmental competence and characterization of the bovine splicing isoforms. Reproduction, 2003;125:437-446. Senger (2012). Pathways to pregnancy and parturition. 3th ed. Redmond: Current Conceptions. Inc., p.102. https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/590/pdf info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/publishedVersion http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Text Publication |
| institution |
UNIVERSIDAD DE LOS LLANOS |
| thumbnail |
https://nuevo.metarevistas.org/UNIVERSIDADDELOSLLANOS/logo.png |
| country_str |
Colombia |
| collection |
Orinoquia |
| title |
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando |
| spellingShingle |
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando Vejarano-Orjuela, Álvaro Hernández-Vásquez, Aureliano Rondón-Barragán, Iang S. Suárez-Mesa, Rafael A. bovine creole reproduction LHR–LHCGR fertility indicator bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR |
| title_short |
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando |
| title_full |
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando |
| title_fullStr |
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando |
| title_full_unstemmed |
Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando |
| title_sort |
polimorfismos del gen del receptor para la hormona luteinizante (lhr – lhgcr) en folículos ováricos de vacas romosinuano y brahman ciclando |
| title_eng |
Luteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cows |
| description |
El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.
|
| description_eng |
The luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance.
|
| author |
Vejarano-Orjuela, Álvaro Hernández-Vásquez, Aureliano Rondón-Barragán, Iang S. Suárez-Mesa, Rafael A. |
| author_facet |
Vejarano-Orjuela, Álvaro Hernández-Vásquez, Aureliano Rondón-Barragán, Iang S. Suárez-Mesa, Rafael A. |
| topic |
bovine creole reproduction LHR–LHCGR fertility indicator bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR |
| topic_facet |
bovine creole reproduction LHR–LHCGR fertility indicator bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR |
| topicspa_str_mv |
bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR |
| citationvolume |
24 |
| citationissue |
1 |
| publisher |
Universidad de los Llanos |
| ispartofjournal |
Orinoquia |
| source |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/590 |
| language |
spa |
| format |
Article |
| rights |
https://creativecommons.org/licenses/by-nc-sa/4.0/ Orinoquia - 2020 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 |
| references |
Cadagan D, Towlson C. Mechanisms of Luteinising Hormone Regulation in Female Steroidogenesis. Am J Med, 2017;5:65-68. Casarini L, Pignatti E, Simoni M. Effects of polymorphisms in gonadotropin and gonadotropin receptor genes on reproductive function. Rev Endocr Metab Dis, 2011;12:303. Clelland E, Peng C. Endocrine/paracrine control of zebrafish ovarian development. Mol Cell Endocrinol, 2009;312:42-52. Delemi A. Identificaion of the expression level to LH-r Gene in dominant and cystic ovarian follicles cells of the cow. Al-Delemi DHJ, Al-Gewary AKA, Ali Jeddoa Z. Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows. J Dairy Vet Anim Res, 2014;1(3):80-86. DOI: 10.15406/jdvar.2014.01.00017 Dickinson RE, Stewart AJ, Myers M, Millar RP Duncan WC. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology, 2009;150:2873-2881. Gay V, Midgley Jr A, Niswender G. (1970). Patterns of gonadotrophin secretion associated with ovulation. Federation proceedings, p. 1880. Ginther O. Selection of the dominant follicle in cattle and horses. Anim Reprod Sci, 2000;60:61-79. Hastings N, Donn S, Derecka K, Flint A, Woolliams J. Polymorphisms within the coding region of the bovine luteinizing hormone receptor gene and their association with fertility traits. Anim Genet, 2006;37:583-585. Holdridge LR. 1967. Life zone ecology. Life zone ecology. Johnson A, Bridgham J, Wagner B. Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod, 1996;55: 304-309. Kawate N. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. J Reprod Develop, 2004;50:1-8. Larsson B. Determination of Ovulation by Ultrasound Examination and its Relation to the LH‐Peak in Heifers. J Vet Med A, 1987;34:749-754. Liu KC, Lin SW, Ge W. Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane. Endocrinology, 2011;152:4418-4430. Ma T, Xiong Q, Yuan B, Jiang H, Gao Y, Xu J, Liu S, et al. Luteinizing hormone receptor splicing variants in bovine Leydig cells. Genet Mol Res, 2012;11:1721-1730. Mamluk R, Wolfenson D, Meidan R. LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin. Domest Anim Endocrinol, 1998;15:103-114. McArdle C, Roberson M. Gonadotropes and gonadotropin-releasing hormone signaling. Knobil and Neill’s Physiology of Reproduction, 2015;4:335-397. Nejad SZ, Tehrani FR, Zadeh-Vakili A. The Role of Kisspeptin in Female Reproduction. Int J Endocrinol Metab, 2017;15(3):e44337. doi: 10.5812/ijem.44337. Nogueira M, Buratini J, Price C, Castilho A, Pinto M, Barros C. Expression of LH receptor mRNA splice variants in bovine granulosa cells: changes with follicle size and regulation by FSH in vitro. Mol Reprod Dev, 2007;74: 680-686. Peiró JR, Nogueira GM, Nogueira GP, Perri SH, Cardoso D. Ovariectomy by left flank approach in prepubertal Nelore (Bos indicus) heifers. Can J Vet Res, 2009;73:237-240. Pierson R, Ginther O. Ultrasonic imaging of the ovaries and uterus in cattle. Theriogenology, 1988;29:21-37. Rawan A, Yoshioka S, Abe H, Acosta T. Insulin‐Like Growth Factor‐1 Regulates the Expression of Luteinizing Hormone Receptor and Steroid Production in Bovine Granulosa Cells. Reprod Domest Anim, 2015;50:283-291. Richards J, Hedin L. Molecular aspects of hormone action in ovarian follicular development, ovulation, and luteinization. Annu Rev Physiol, 1988;50: 441-463. Robert C, Gagne D, Lussier J, Bousquet D, Barnes F, Sirard M. Presence of LH receptor mRNA in granulosa cells as a potential marker of oocyte developmental competence and characterization of the bovine splicing isoforms. Reproduction, 2003;125:437-446. Senger (2012). Pathways to pregnancy and parturition. 3th ed. Redmond: Current Conceptions. Inc., p.102. |
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