Titulo:
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed
.
Sumario:
ASBTRACT: The Murciano-levantina cattle from the Spanish South-East belongs to a bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity, longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work was to deter mine the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3 µg/mL LH and µg/mL FSH. Oocytes presumed matured were fertilized with Percoll-prepared sp... Ver más
Guardado en:
0121-3709
2011-2629
8
2004-01-01
25
25
Orinoquia - 2014
info:eu-repo/semantics/openAccess
http://purl.org/coar/access_right/c_abf2
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metarevistapublica_unillanos_orinoquia_57_article_186 |
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Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed ASBTRACT: The Murciano-levantina cattle from the Spanish South-East belongs to a bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity, longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work was to deter mine the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3 µg/mL LH and µg/mL FSH. Oocytes presumed matured were fertilized with Percoll-prepared sper m (750.000/mL) in IVF-TALP as described by Parrish et al. (Biol. Reprod 38:1171-1180, 1988). Zygotes (at 18 h) or two-cell stage embryos (at 48 h) were assessed by staining with Hoechst 33342 (µg/mL; 20 min) under an epifluorescence microscopy. The experiment was replicated on 4 occasions.The results of penetration (PEN-%), average number of spermato- zoa inside the oocyte (S/O), monos permy (MON- %) putative embryos (zygotes with two pronuclei, 2 PN-%) and percentages of cleavage at 48 hours (Table 1) were recorded. The results show that early embryos can be obtained with no difference with the source of sperm used in the monospermy and the cleavage rate between commercial and Mur- ciano-levantina sperm (p>0.05). *Lista Mundial de Vigilancia para la Diversidad de los Animales Do- mésticos (1997). http:// www.fao.org/docrep/V8300S/ V8300S00.htm Barajas, DP Velásquez, JG and Coy P, Poto A 8 1 Artículo de revista Journal article 2004-01-01T00:00:00Z 2004-01-01T00:00:00Z 2004-01-01 application/msword application/pdf text/html Universidad de los Llanos Orinoquia 0121-3709 2011-2629 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/186 10.22579/20112629.186 https://doi.org/10.22579/20112629.186 spa https://creativecommons.org/licenses/by-nc-sa/4.0/ Orinoquia - 2014 25 25 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/341 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/342 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/643 info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/publishedVersion http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Text Publication |
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UNIVERSIDAD DE LOS LLANOS |
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https://nuevo.metarevistas.org/UNIVERSIDADDELOSLLANOS/logo.png |
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Colombia |
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Orinoquia |
| title |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| spellingShingle |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed Barajas, DP Velásquez, JG and Coy P, Poto A |
| title_short |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| title_full |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| title_fullStr |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| title_full_unstemmed |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| title_sort |
preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| title_eng |
Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed |
| description |
ASBTRACT: The Murciano-levantina cattle from the Spanish South-East belongs to a bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity, longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work was to deter mine the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3 µg/mL LH and µg/mL FSH. Oocytes presumed matured were fertilized with Percoll-prepared sper m (750.000/mL) in IVF-TALP as described by Parrish et al. (Biol. Reprod 38:1171-1180, 1988). Zygotes (at 18 h) or two-cell stage embryos (at 48 h) were assessed by staining with Hoechst 33342 (µg/mL; 20 min) under an epifluorescence microscopy. The experiment was replicated on 4 occasions.The results of penetration (PEN-%), average number of spermato- zoa inside the oocyte (S/O), monos permy (MON- %) putative embryos (zygotes with two pronuclei, 2 PN-%) and percentages of cleavage at 48 hours (Table 1) were recorded. The results show that early embryos can be obtained with no difference with the source of sperm used in the monospermy and the cleavage rate between commercial and Mur- ciano-levantina sperm (p>0.05). *Lista Mundial de Vigilancia para la Diversidad de los Animales Do- mésticos (1997). http:// www.fao.org/docrep/V8300S/ V8300S00.htm
|
| author |
Barajas, DP Velásquez, JG and Coy P, Poto A |
| author_facet |
Barajas, DP Velásquez, JG and Coy P, Poto A |
| citationvolume |
8 |
| citationissue |
1 |
| publisher |
Universidad de los Llanos |
| ispartofjournal |
Orinoquia |
| source |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/186 |
| language |
spa |
| format |
Article |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ Orinoquia - 2014 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
| publishDate |
2004-01-01 |
| date_accessioned |
2004-01-01T00:00:00Z |
| date_available |
2004-01-01T00:00:00Z |
| url |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/186 |
| url_doi |
https://doi.org/10.22579/20112629.186 |
| issn |
0121-3709 |
| eissn |
2011-2629 |
| doi |
10.22579/20112629.186 |
| citationstartpage |
25 |
| citationendpage |
25 |
| url2_str_mv |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/342 |
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https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/643 |
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