Involvement of sialic acid in bovine sperm-zona pellucida interaction
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ABSTRAC: perm-egg interaction is a complex process mediated by receptors and ligands present in both gametes, spermatozoon and oocyte. The important role played by the carbohydrates in the gamete interaction is well documented. However, the identification of the molecules involved in this process remains unsolved. A carbohydrate modification in the sialic acid contents of the bovine zona pellucida (ZP) post fertilization has been previously reported. However, no evidence exists on the implication of this carbohydrate in the sperm-ZP interaction. In this study, we investigate the role played by the sialic acid residues in the sperm-ZP binding by means of different sperm binding competition and IVF assays with different sialic acid spec... Ver más
0121-3709
2011-2629
8
2004-01-01
24
24
Orinoquia - 2014
info:eu-repo/semantics/openAccess
http://purl.org/coar/access_right/c_abf2
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Involvement of sialic acid in bovine sperm-zona pellucida interaction Involvement of sialic acid in bovine sperm-zona pellucida interaction ABSTRAC: perm-egg interaction is a complex process mediated by receptors and ligands present in both gametes, spermatozoon and oocyte. The important role played by the carbohydrates in the gamete interaction is well documented. However, the identification of the molecules involved in this process remains unsolved. A carbohydrate modification in the sialic acid contents of the bovine zona pellucida (ZP) post fertilization has been previously reported. However, no evidence exists on the implication of this carbohydrate in the sperm-ZP interaction. In this study, we investigate the role played by the sialic acid residues in the sperm-ZP binding by means of different sperm binding competition and IVF assays with different sialic acid specific lectins, neuraminidase enzyme and antibodies. Velásquez, J. G Marcos, J Gardón, JC Jiménez-Movilla, M Ballesta, J Coy, P Avilés, M 8 1 Artículo de revista Journal article 2004-01-01T00:00:00Z 2004-01-01T00:00:00Z 2004-01-01 application/msword application/pdf text/html Universidad de los Llanos Orinoquia 0121-3709 2011-2629 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/181 10.22579/20112629.181 https://doi.org/10.22579/20112629.181 spa https://creativecommons.org/licenses/by-nc-sa/4.0/ Orinoquia - 2014 24 24 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/331 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/332 https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/642 info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/publishedVersion http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Text Publication |
institution |
UNIVERSIDAD DE LOS LLANOS |
thumbnail |
https://nuevo.metarevistas.org/UNIVERSIDADDELOSLLANOS/logo.png |
country_str |
Colombia |
collection |
Orinoquia |
title |
Involvement of sialic acid in bovine sperm-zona pellucida interaction |
spellingShingle |
Involvement of sialic acid in bovine sperm-zona pellucida interaction Velásquez, J. G Marcos, J Gardón, JC Jiménez-Movilla, M Ballesta, J Coy, P Avilés, M |
title_short |
Involvement of sialic acid in bovine sperm-zona pellucida interaction |
title_full |
Involvement of sialic acid in bovine sperm-zona pellucida interaction |
title_fullStr |
Involvement of sialic acid in bovine sperm-zona pellucida interaction |
title_full_unstemmed |
Involvement of sialic acid in bovine sperm-zona pellucida interaction |
title_sort |
involvement of sialic acid in bovine sperm-zona pellucida interaction |
title_eng |
Involvement of sialic acid in bovine sperm-zona pellucida interaction |
description |
ABSTRAC: perm-egg interaction is a complex process mediated by receptors and ligands present in both gametes, spermatozoon and oocyte. The important role played by the carbohydrates in the gamete interaction is well documented. However, the identification of the molecules involved in this process remains unsolved. A carbohydrate modification in the sialic acid contents of the bovine zona pellucida (ZP) post fertilization has been previously reported. However, no evidence exists on the implication of this carbohydrate in the sperm-ZP interaction. In this study, we investigate the role played by the sialic acid residues in the sperm-ZP binding by means of different sperm binding competition and IVF assays with different sialic acid specific lectins, neuraminidase enzyme and antibodies.
|
author |
Velásquez, J. G Marcos, J Gardón, JC Jiménez-Movilla, M Ballesta, J Coy, P Avilés, M |
author_facet |
Velásquez, J. G Marcos, J Gardón, JC Jiménez-Movilla, M Ballesta, J Coy, P Avilés, M |
citationvolume |
8 |
citationissue |
1 |
publisher |
Universidad de los Llanos |
ispartofjournal |
Orinoquia |
source |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/181 |
language |
spa |
format |
Article |
rights |
https://creativecommons.org/licenses/by-nc-sa/4.0/ Orinoquia - 2014 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 |
type_driver |
info:eu-repo/semantics/article |
type_coar |
http://purl.org/coar/resource_type/c_6501 |
type_version |
info:eu-repo/semantics/publishedVersion |
type_coarversion |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
type_content |
Text |
publishDate |
2004-01-01 |
date_accessioned |
2004-01-01T00:00:00Z |
date_available |
2004-01-01T00:00:00Z |
url |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/181 |
url_doi |
https://doi.org/10.22579/20112629.181 |
issn |
0121-3709 |
eissn |
2011-2629 |
doi |
10.22579/20112629.181 |
citationstartpage |
24 |
citationendpage |
24 |
url2_str_mv |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/332 |
url3_str_mv |
https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/642 |
_version_ |
1811200613540691968 |