Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
Titulo:

Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
.

Sumario:

Introducción: Los polifenoles son compuestos que se encuentran naturalmente en alimentos como frutas, verduras, té, vino y chocolates, a los que se les atribuyen beneficios a la salud humana por su capacidad antioxidante. El cáncer de las vías digestivas se encuentra entre la tercera y quinta causas de muerte para la población, por lo que se ha incrementado el interés por realizar los estudios dirigidos a encontrar compuestos polifenólicos que ayuden en su prevención o tratamiento. Objetivo: Identificar las estrategias disponibles para la evaluación de polifenoles en células de cáncer de vías digestivas. Metodología: Búsqueda de literatura en bases de datos como Ovid, Pubmed, Science Direct y Elsevier Journal. Se seleccionaron artículos en... Ver más

Guardado en:

Revista Investigación en Salud Universidad de Boyacá

2389-7325

2539-2018

Ferrebuz-Cardozo, Atilio Junior

Sánchez-Quitian, Zilpa Adriana

Márquez-Salcedo, Ruby Alba Elizabeth

Carreño-Saltarén, Lady Johanna

UNIVERSIDAD DE BOYACÁ

10.24267/23897325.916

9

2022-08-29

194

213

Colombia

extractos vegetales

polifenoles

neoplasias

tracto gastrointestinal

técnicas in vitro

capacidad de absorbancia de radicales de oxígeno

concentración inhibitoria 50

apoptosis

La Revista Investigación en Salud (ISUB) es una publicación que sigue los términos de la licencia Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) la cual permite con una adecuada citación que los manuscritos sean usados de forma ilimitada; esta publicación se declara como de acceso abierto. Se conservan los derechos de autoría de los investigadores sobre su artículo; mientras que los derechos comerciales de publicación y los derechos para la publicación de compilaciones o traducciones de los manuscritos los conserva la Revista ISUB. Los datos personales de los autores que figuran en los manuscritos (nombres, direcciones de correo, ORCID y filiación) serán usados únicamente para los fines declarados de divulgación científica por la revista. - 2022

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.

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spelling Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
Strategies for Evaluation of Polyphenol Extracts on in vitro Models of Digestive Tract Cancer
Introducción: Los polifenoles son compuestos que se encuentran naturalmente en alimentos como frutas, verduras, té, vino y chocolates, a los que se les atribuyen beneficios a la salud humana por su capacidad antioxidante. El cáncer de las vías digestivas se encuentra entre la tercera y quinta causas de muerte para la población, por lo que se ha incrementado el interés por realizar los estudios dirigidos a encontrar compuestos polifenólicos que ayuden en su prevención o tratamiento. Objetivo: Identificar las estrategias disponibles para la evaluación de polifenoles en células de cáncer de vías digestivas. Metodología: Búsqueda de literatura en bases de datos como Ovid, Pubmed, Science Direct y Elsevier Journal. Se seleccionaron artículos en los cuales se reporta el efecto biológico de los polifenoles sobre líneas celulares de cáncer de vías digestivas publicados entre 2012 y 2022. Resultados: Varios estudios han reportado el uso de un buen número de líneas celulares como modelos in vitro para estudios de polifenoles en cáncer y han resaltado las líneas AGS y HT-29, además de técnicas para la caracterización de los polifenoles, como el ensayo 2,2-Difenil-I-Picril Hidrazilo (DPPH). Sin embargo, para evaluar el efecto biológico se identifican diversas pruebas que deben analizarse antes de su implementación. Conclusiones: En la literatura se identifica que existen varias alternativas y estrategias para la evaluación de extractos vegetales en cultivos in vitro de cáncer de vías digestivas; no obstante, antes de pasar al diseño experimental, deben tenerse en cuenta una serie de consideraciones para garantizar la utilidad de los resultados.
Introduction: Polyphenols are compounds naturally found in foods such as fruits, vegetables, tea, wine and chocolates, and it was attributed with benefits to human health due to their antioxidant capacity. Cancer of the digestive tract is between the third and fifth cause of death for the population, increasing the interest in carrying out studies aimed at finding polyphenolic compounds that help in their prevention or treatment. Objective: Identify the available strategies for the evaluation of polyphenols in digestive tract cancer cells. Method: A literature search was performed in databases such Ovid, Pubmed, Science Direct and Elsevier Journal and selected articles reporting the biological effect of polyphenols on digestive tract cancer cell lines, published between 2012 and 2022. Results: Currently studies report the use of a good number of cell lines as in vitro models for polyphenol studies in cancer highlighting the AGS and HT-29 lines, in addition to techniques for the characterization of polyphenols such as the α, α-diphenyl-β-picrylhydrazyl DPPH assay, however, to evaluate the biological effect various tests are identified that should be analyzed before implementation. Conclusions: The literature identifies that there are many alternatives and strategies for the evaluation of plant extracts in in vitro cultures of digestive tract cancer, however, before moving on to the experimental design, a number of considerations should be taken into account to ensure the usability of the results
Ferrebuz-Cardozo, Atilio Junior
Sánchez-Quitian, Zilpa Adriana
Márquez-Salcedo, Ruby Alba Elizabeth
Carreño-Saltarén, Lady Johanna
extractos vegetales
polifenoles
neoplasias
tracto gastrointestinal
técnicas in vitro
capacidad de absorbancia de radicales de oxígeno
concentración inhibitoria 50
apoptosis
plant extracts
polyphenols
neoplasms
gastrointestinal tract
in vitro techniques
oxygen radical absorbance capacity
inhibitory concentration 50
apoptosis
Extratos Vegetais
Polifenóis
Neoplasias
Trato Gastrointestinal
Técnica in vitro
Capacidade de Absorbância de Radicais de Oxigênio
Concentração Inibidora 50
apoptose
9
2
Núm. 2 , Año 2022 : Revista Investigación en Salud Universidad de Boyacá
Artículo de revista
Journal article
2022-08-29T00:00:00Z
2022-08-29T00:00:00Z
2022-08-29
application/pdf
text/xml
Universidad de Boyacá
Revista Investigación en Salud Universidad de Boyacá
2389-7325
2539-2018
https://revistasdigitales.uniboyaca.edu.co/index.php/rs/article/view/916
10.24267/23897325.916
https://doi.org/10.24267/23897325.916
spa
http://creativecommons.org/licenses/by-nc/4.0
La Revista Investigación en Salud (ISUB) es una publicación que sigue los términos de la licencia Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) la cual permite con una adecuada citación que los manuscritos sean usados de forma ilimitada; esta publicación se declara como de acceso abierto. Se conservan los derechos de autoría de los investigadores sobre su artículo; mientras que los derechos comerciales de publicación y los derechos para la publicación de compilaciones o traducciones de los manuscritos los conserva la Revista ISUB. Los datos personales de los autores que figuran en los manuscritos (nombres, direcciones de correo, ORCID y filiación) serán usados únicamente para los fines declarados de divulgación científica por la revista. - 2022
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.
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https://revistasdigitales.uniboyaca.edu.co/index.php/rs/article/download/916/792
https://revistasdigitales.uniboyaca.edu.co/index.php/rs/article/download/916/855
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Text
Publication
institution UNIVERSIDAD DE BOYACÁ
thumbnail https://nuevo.metarevistas.org/UNIVERSIDADDEBOYACA/logo.png
country_str Colombia
collection Revista Investigación en Salud Universidad de Boyacá
title Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
spellingShingle Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
Ferrebuz-Cardozo, Atilio Junior
Sánchez-Quitian, Zilpa Adriana
Márquez-Salcedo, Ruby Alba Elizabeth
Carreño-Saltarén, Lady Johanna
extractos vegetales
polifenoles
neoplasias
tracto gastrointestinal
técnicas in vitro
capacidad de absorbancia de radicales de oxígeno
concentración inhibitoria 50
apoptosis
plant extracts
polyphenols
neoplasms
gastrointestinal tract
in vitro techniques
oxygen radical absorbance capacity
inhibitory concentration 50
apoptosis
Extratos Vegetais
Polifenóis
Neoplasias
Trato Gastrointestinal
Técnica in vitro
Capacidade de Absorbância de Radicais de Oxigênio
Concentração Inibidora 50
apoptose
title_short Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
title_full Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
title_fullStr Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
title_full_unstemmed Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
title_sort estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas
title_eng Strategies for Evaluation of Polyphenol Extracts on in vitro Models of Digestive Tract Cancer
description Introducción: Los polifenoles son compuestos que se encuentran naturalmente en alimentos como frutas, verduras, té, vino y chocolates, a los que se les atribuyen beneficios a la salud humana por su capacidad antioxidante. El cáncer de las vías digestivas se encuentra entre la tercera y quinta causas de muerte para la población, por lo que se ha incrementado el interés por realizar los estudios dirigidos a encontrar compuestos polifenólicos que ayuden en su prevención o tratamiento. Objetivo: Identificar las estrategias disponibles para la evaluación de polifenoles en células de cáncer de vías digestivas. Metodología: Búsqueda de literatura en bases de datos como Ovid, Pubmed, Science Direct y Elsevier Journal. Se seleccionaron artículos en los cuales se reporta el efecto biológico de los polifenoles sobre líneas celulares de cáncer de vías digestivas publicados entre 2012 y 2022. Resultados: Varios estudios han reportado el uso de un buen número de líneas celulares como modelos in vitro para estudios de polifenoles en cáncer y han resaltado las líneas AGS y HT-29, además de técnicas para la caracterización de los polifenoles, como el ensayo 2,2-Difenil-I-Picril Hidrazilo (DPPH). Sin embargo, para evaluar el efecto biológico se identifican diversas pruebas que deben analizarse antes de su implementación. Conclusiones: En la literatura se identifica que existen varias alternativas y estrategias para la evaluación de extractos vegetales en cultivos in vitro de cáncer de vías digestivas; no obstante, antes de pasar al diseño experimental, deben tenerse en cuenta una serie de consideraciones para garantizar la utilidad de los resultados.
description_eng Introduction: Polyphenols are compounds naturally found in foods such as fruits, vegetables, tea, wine and chocolates, and it was attributed with benefits to human health due to their antioxidant capacity. Cancer of the digestive tract is between the third and fifth cause of death for the population, increasing the interest in carrying out studies aimed at finding polyphenolic compounds that help in their prevention or treatment. Objective: Identify the available strategies for the evaluation of polyphenols in digestive tract cancer cells. Method: A literature search was performed in databases such Ovid, Pubmed, Science Direct and Elsevier Journal and selected articles reporting the biological effect of polyphenols on digestive tract cancer cell lines, published between 2012 and 2022. Results: Currently studies report the use of a good number of cell lines as in vitro models for polyphenol studies in cancer highlighting the AGS and HT-29 lines, in addition to techniques for the characterization of polyphenols such as the α, α-diphenyl-β-picrylhydrazyl DPPH assay, however, to evaluate the biological effect various tests are identified that should be analyzed before implementation. Conclusions: The literature identifies that there are many alternatives and strategies for the evaluation of plant extracts in in vitro cultures of digestive tract cancer, however, before moving on to the experimental design, a number of considerations should be taken into account to ensure the usability of the results
author Ferrebuz-Cardozo, Atilio Junior
Sánchez-Quitian, Zilpa Adriana
Márquez-Salcedo, Ruby Alba Elizabeth
Carreño-Saltarén, Lady Johanna
author_facet Ferrebuz-Cardozo, Atilio Junior
Sánchez-Quitian, Zilpa Adriana
Márquez-Salcedo, Ruby Alba Elizabeth
Carreño-Saltarén, Lady Johanna
topicspa_str_mv extractos vegetales
polifenoles
neoplasias
tracto gastrointestinal
técnicas in vitro
capacidad de absorbancia de radicales de oxígeno
concentración inhibitoria 50
apoptosis
topic extractos vegetales
polifenoles
neoplasias
tracto gastrointestinal
técnicas in vitro
capacidad de absorbancia de radicales de oxígeno
concentración inhibitoria 50
apoptosis
plant extracts
polyphenols
neoplasms
gastrointestinal tract
in vitro techniques
oxygen radical absorbance capacity
inhibitory concentration 50
apoptosis
Extratos Vegetais
Polifenóis
Neoplasias
Trato Gastrointestinal
Técnica in vitro
Capacidade de Absorbância de Radicais de Oxigênio
Concentração Inibidora 50
apoptose
topic_facet extractos vegetales
polifenoles
neoplasias
tracto gastrointestinal
técnicas in vitro
capacidad de absorbancia de radicales de oxígeno
concentración inhibitoria 50
apoptosis
plant extracts
polyphenols
neoplasms
gastrointestinal tract
in vitro techniques
oxygen radical absorbance capacity
inhibitory concentration 50
apoptosis
Extratos Vegetais
Polifenóis
Neoplasias
Trato Gastrointestinal
Técnica in vitro
Capacidade de Absorbância de Radicais de Oxigênio
Concentração Inibidora 50
apoptose
citationvolume 9
citationissue 2
citationedition Núm. 2 , Año 2022 : Revista Investigación en Salud Universidad de Boyacá
publisher Universidad de Boyacá
ispartofjournal Revista Investigación en Salud Universidad de Boyacá
source https://revistasdigitales.uniboyaca.edu.co/index.php/rs/article/view/916
language spa
format Article
rights http://creativecommons.org/licenses/by-nc/4.0
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